17.T7 RNA Polymerase Hybrid System - nshtvn.org
Using liposome-mediated transfection, this recombi- ... OST7-1 cells (a stable cell line that constitutively expresses the T7 RNA ... Current Protocols in Protein Science (1998) 5.15.1-5.15.11 ... tion or PEG precipitation (APPENDIX 4C). ..... If a fusion protein is desired, the ... and immunological properties of human immu- ...
PS0515
www.nshtvn.org 
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18.Cell fusion of bone marrow cells and somatic cell reprogramming by ...
Sep 17, 2009 ... Advantage Program, and ‡Immunology Graduate Program, ... In contrast, after cell fusion with embryonic stem cells, .... The protocols for .... such as PEG, which has been previously applied to .... cells, Cre-mediated recombination would excise the .... The current data not only provide evidence that fused ...
FJ.09-137141.FULL
www.fasebj.org 21
19.activation of antituMor cytotoxic t lyMphocytes by fusion of patient ...
Jul 17, 2005 ... of mice with fusion cells has induced protective and therapeutic ... traditional fusion method using polyethylene glycol .... For the current study, the .... In this study, an electrofusion protocol was em- ... cells: immunobiology and cancer immunotherapy. ... tric field-mediated and PEG-mediated cell fusion for the ...
457?UPLOAD=
exp-oncology.com.ua 0
20.ENHANCED ANTITUMOR IMMUNITY DERIVED FROM A NOVEL ...
Aim: Dendritic cell — tumor cell fusion hybrid vaccines which facilitate ... that the current conventional protocol of simply fusing dendritic cells (DCs) and ... (CD40L ) molecule using polyethylene glycol (PEG). ... immunity derived from vaccination of DC/J558CD40L was mainly mediated by ..... Immunology 2000; 101: 316–24.
371?UPLOAD=
exp-oncology.com.ua 0
21.Cell-surface expression of a mutated Epstein–Barr virus glycoprotein B
Dec 14, 2004 ... Department of Microbiology and Immunology, The Feinberg School of Medicine, Northwestern University, Chicago, IL 60611 ... allowed gB-mediated fusion with epithelial cells, albeit at a lower ... addition of polyethylene glycol at the nonpermissive tempera- .... manufacturer's protocol (Molecular Probes).
17474.FULL
www.pnas.org 506
22.characterization of the NeS2Y cell line and its use in the production ...
a current area of great scientific interest but must, at present, be regarded to be in its ... Biology, Phisiology and Immunology; Universitat Autonoma de Barcelona; Bellaterra, Barcelona Spain ... nies when PEG-mediated fusion was used indicating that elec- ... fusion protocols with NES2Y cells and normal human islet β-cells ...
ADAMSISLETS1-2
www.landesbioscience.com 0
23.Bispecific Antibodies from Hybrid Hybridoma
immunological effector cells or molecules toward tumor cells. Targeting an .... After PEG fusion only hybrid hybridoma cells can actively grow in HAT medium ...
9783642209093-C1?SGWID=0-0-45-1164442-P174120860
www.springer.com 120
24.Exposure to the electrofusion process can increase the ...
of allogeneic human cells, in particular the colorectal cell line, SW620. ... suggest that EF protocols should be evaluated with re- gard to the ... Chemical fusion using polyethlene glycol (PEG) is the traditional ... Cancer Immunol Immunother ( 2005) 54: 880–890 .... aligned by application of a direct current of 25 V sup- plied for ...
AMCL_15
micro.otago.ac.nz 0
